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KMID : 0369820140440070473
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Jorunal of Korean Pharmaceutical Sciences
2014 Volume.44 No. 7 p.473 ~ p.483
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Angiopep-2-conjugated liposomes encapsulating γ-secretase inhibitor for targeting glioblastoma stem cells
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Xuan Shu Hua
Shin Dae-Hwan
Kim Jin-Seok
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Abstract
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CD133+ cell subpopulation in U87 MG cells displays glioblastoma stem cell (GSC) like properties. Notch, a key regulator of stem cells, is also over-activated in GSCs. It was previously reported that γ-secretase inhibitors inhibit the Notch pathway by targeting the γ-secretase complex. To examine the GSCs, CD133+ cells were separated from the U87 MG cells by magnetic activated cell sorting and only cell populations with nearly 90 % CD133+ cells were used as GSCs. The sorted U87-CD133+ cells indeed display enhanced chemoresistance and high Notch activity. For targeting studies, Angiopep-2 (An2)-conjugated YO-01027 (YO) encapsulating liposomes (PEG-lipo-YO-An2) were prepared by lipid film hydration method. An2 is an effective ligand of low density lipoprotein receptor-related protein which is over-expressed in the blood?brain barrier and GSCs. The mean diameter, zeta potential and encapsulation efficiency of PEG-lipo-YO-An2 was around 180 nm, ?10.0 mV and 56.6 %, respectively. In in vitro studies, we confirmed that PEG-lipo-YO-An2 showed enhanced anti-GSC properties such as enhanced stability, anti-proliferation and anti-tumor sphere formation abilities towards than free drug. This study demonstrates that An2 conjugation and liposomal encapsulation of YO enhance the cytotoxicity of YO against GSCs, and this formulation could be used as a promising candidate for the treatment of glioblastoma multiforme by targeting GSCs.
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KEYWORD
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γ-Secretase inhibitor, CD133, Angiopep-2, Glioblastoma multiforme, Liposome
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